In this article we will discuss about the Antibiotic Sensitivity Test of Bacteria. Subcultures of the test organisms are made in broth and incubated for 6-18 hours. Any density of inoculum may be used but in most cases the surfaces of cultures plates may be sown with broth containing approximately 100 million cells per ml. After drying the surface, the [...]
In this article we will discuss about Streptococci:- 1. Meaning of Streptococci 2. Morphology and Staining of Streptococci 3. Cultural Characteristics 4. Biochemical Reaction 5. Antigenic Structure 6. Toxins and Enzymes 7. Haemolysin 8. Infective Endocarditis 9. Sub-Acute Endocarditis 10. Post-Streptococcal Disease 11. Laboratory Diagnosis 12. Serological Tests 13. Treatment. Contents: Meaning of Streptococci Morphology and Staining of Streptococci Cultural [...]
The following points highlight the top three staining methods used for colouring the biological materials. The types are: 1. Negative Staining 2. Simple Staining 3. Differential Staining. Method # 1. Negative Staining: For quicker observation of the morphology of bacterial or yeast cells, the negative staining technique is applied. Requirements: 1. Bacterial/yeast suspension. 2. Slide, inoculating needle, pipettes etc. 3. [...]