In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: Prepare a fresh solution by [...]
A number of inorganic elements are present in plants which also catalyse many enzymatic reactions. The existence of such inorganic elements in plants can be detected by the chemical tests of plant ash. Requirements for Detection of Ash: (a) Preparation of Ash Extract: For detection of various elements in plant ash viz., Ca, Mg, Fe, S, P, CI etc. ash [...]
In this article we will discuss about the isolation of pigments and their characterization. Theory of Plant Pigments: Plant pigments are mostly the colouring substances found either in cell sap (as water-soluble forms, or in the plastids (water-insoluble forms). Normally, the plastidial pigments are responsible for photosynthesis while cell sap pigments are responsible for coloration of floral parts and various [...]