In this article we will discuss about the antisense RNA in post harvesting improvement of plants. Engineering of Fruit Ripening Process: Efficacy of antisense RNA technology in post harvesting has been implicated well in extending shelf life of fruits, vegetables and vase life of flowers. Infact the first genetically modified products commercialized in markets were calgenes flavr savr tomatoes. Antisense [...]
Molecular markers are DNA sequences, whose inheritance pattern can be established. Classic examples of molecular markers are: 1. Restriction Fragment Length Polymorphism (RFLP) 2. Randomly Amplified Polymorphic DNA (RAPD) 3. Amplified Fragments Length Polymorphism (AFLP) 4. Sequence Characterized Amplified Region (SCAR). Example # 1. Restriction Fragment Length Polymorphism (RFLP): This novel non-PCR based approach is based on hybridization of probe [...]
In this article we will discuss about the mechanism of antisense ribosome. Persistent search for novel vehicle for introducing antisense RNA into cells has become reality when Sweeney and Chaoyao (1998) developed antisense ribosomes in Tetrahymena thermophila. This system allows expression of antisense gene present with rRNA genes and antisense RNA alters expression function as a part of a stable [...]