Get the answer of: Are all cells in culture totipotent? A callus mass contains thousands of cells. Theoretically, if the totipotency of all cells are expressed at a time, then it is expected that equal number of shoots or roots or embryoids will be regenerated from such totipotent cells. But in experiment, such results are not obtained. The reasons behind [...]
In the below mentioned article, we will discuss about the growth patterns in cell suspension culture and the measurement of cell growth in cell suspension culture. Growth Patterns in Cell Suspension Culture: Under appropriate light, temperature, aeration and nutrient medium the growth of suspension culture follows a predictable pattern or growth curve. The growth of suspension culture can be monitored [...]
The below mentioned article provides a general account of suspension culture. Suspension culture can be initiated either from pre-established callus culture or from the explant directly into liquid medium which is continuously agitated on a moving elliptical or rotary shaker. The movement of the nutrient medium provides vital aeration of the medium to sustain cell respiration in the liquid medium [...]