The following points highlight the top six methods used for obtaining pure culture of microorganisms. The methods are: 1. Streak Plate Method 2. Pour Plate Method 3. Spread Plate Method 4. Serial Dilution Method 5. Single Cell Isolation Methods 6. Enrichment Culture Method. 1. Streak Plate Method: This method is used most commonly to isolate pure cultures of bacteria. A [...]
A pure culture of an organism is a valuable asset and it deserves to be preserved for future. Normally, the cultures which are of daily use are grown in agar slants and kept in the refrigerator. When required, a loopful of growth is transferred to fresh agar or broth and the organisms are allowed to grow in an incubator. For [...]
The below mentioned article provides notes on the Krebs’ Cycle. The TCA cycle or Krebs' cycle (after H. A. Krebs) is a cyclic sequence of reactions through which pyruvic acid produced in the EMP and EDP is oxidized. The cycle operates in aerobic organisms including animals, plants and microorganisms. The main function of the cycle is to generate energy by [...]