The following points highlight the top two methods for clotting time. The methods are: 1. Capillary Tube Method 2. Lee and White Method. 1. Capillary Tube Method: Procedure: i. Clean the tip of a finger with spirit. ii. Puncture it upto 3 mm deep with a disposable needle. iii. Start the stopwatch. iv. Fill two capillary tube with free flowing [...]
The following points highlight the top four indices of red blood cells. The indices are: 1. Mean Cell Volume 2. Increased MCV 3. Decreased MCV 4. Mean Cell Hemoglobin. 1. Mean Cell Volume (MCV): The mean cell volume indicates the volume of the "average" red cell in a sample. It is expressed in femtoliters (fl; 10-15 liters). Traditionally, MCV was [...]
Whole blood is diluted with a 3% acetic acid solution, which hemolyzes mature erythrocytes and facilitates leukocyte counting. The standard dilution for leukocyte counts is 1:20. This dilution is prepared using the leukocyte Unopette system. The dilution is mixed well and incubated to permit lysis of the erythrocytes. Following the incubation period, the dilution is mounted on a hemocytometer. The [...]