In this article we will discuss about the genetic map of E.coli. Several methods have revealed that the genetic map of the main chromosome of E. coli is an un-branched circle with a circumference of 100 minutes (Fig. 17.5) which corresponds to the transfer time during conjugation. About 650 genes have been mapped. Some important features of the genetic map [...]
A genome library consists of a large collection of bacterial cultures, in each of which a different segment of the genomic DNA of an organism has been cloned by recombinant DNA technology, so that the entire collection represents its complete genome. The analogy between a library of books and a genome library is based on the fact that both genes [...]
In this article we will discuss about Modern Genetic Mapping:- 1. Introduction to Genetic Mapping 2. Methods of Genetic Mapping 3. Isolation of Complementary DNA (cDNA) 4. Recombinant DNA Technology 5. Cloning Vectors and their Nature 6. Shuttle Vector 7. Genomic Libraries. Contents: Introduction to Genetic Mapping Methods of Genetic Mapping Isolation of Complementary DNA (cDNA) Recombinant DNA Technology Cloning [...]