The possibility of sequencing an entire chromosome was first discussed in 1982 and the Human Genome Project dates back to 1986. Why this desire for ever longer genome sequences? There are following reasons for this urge: 1. Significance of Knowledge of the Complete Genetic Content of a Genome 2. Access to Important Genes 3. Identification of Genes for Human Diseases [...]
The following points highlight the three ways by which sequencing of genes in isolation from rest of human genome takes place. The ways are: 1. Complementary DNA (cDNA) Sequencing 2. Using CpG Island of Locate Genes 3. DNA Sequencing for Wheat Genome. Way # 1. Complementary DNA (cDNA) Sequencing: cDNA is made by converting the mRNA content of cell is [...]
Geneticists use following two methods for sequencing the genomes: 1. Clone-by-clone Method 2. Shotgun Cloning. Method # 1. Clone-by-Clone Method: This method was the first to be developed. It begins with the construction of genomic libraries of restriction fragments covering all the genomic DNA (or genomic clones) of an organism. By using genetic markers, overlapping clones are assembled into genetic [...]