The cloning of single genes is usually best carried out using plasmids, since the insert will rarely be larger than about 2 kb. However, for cloning of larger pieces of DNA (e.g., during gene library construction) these plasmids are not suitable as larger inserts increase the plasmid size, making the transformation inefficient. Large DNA molecules can be injected in host [...]
The following points highlight the three important techniques for genome sequencing. The techniques are: 1. Shotgun Approach for Small Bacterial Genome 2. Sequencing of Mapped Clones for S.Cerevisiae Genome 3. Mapping and Sequencing the Human Genome. Technique # 1. Shotgun Approach for Small Bacterial Genome: The first bacterial sequence to be published, for the 1830 kb genome of the bacterium [...]
The possibility of sequencing an entire chromosome was first discussed in 1982 and the Human Genome Project dates back to 1986. Why this desire for ever longer genome sequences? There are following reasons for this urge: 1. Significance of Knowledge of the Complete Genetic Content of a Genome 2. Access to Important Genes 3. Identification of Genes for Human Diseases [...]