In this article we will discuss about the meaning of transgenic mice. Cloned genes can be introduced into the germ line cells of an animal allowing study of the intact animal rather than in cultured cells. For this purpose, the nucleus in mouse embryonal cells has been used for injecting foreign DNA. The goal in such experiments is to have [...]
In this article we will discuss about the definition and strategies of genetic engineering. Definition of Genetic Engineering: The deliberate modification in genetic material of an organism by changing the nucleic acid directly is called genetic engineering. Genetic engineering holds the potential to extend the range and power of every aspect of biotechnology. The techniques will be used widely to [...]
The following points highlight the five main enzymes involved in genetic engineering. The enzymes are: 1. Restriction Endonuclease 2. DNA Ligase 3. Alkaline Phosphatase 4. DNA Polymerase and the Klenow Fragment 5. Reverse Transcriptase. Genetic engineering became possible with the discovery of mainly two types of enzymes: the cutting enzymes called restriction endonucleases and the joining enzymes called ligases. Restriction [...]