In this article we will discuss about the role of Ti plasmids in genetic engineering of plants. Genetic engineering of plants is carried out by introducing DNA into a cell in culture that can grow into a mature plant. An efficient vector for introduction of recombinant DNA into plant cells has been developed from plant viruses. Furthermore, the Ti plasmid [...]
In this article we will discuss about the definition and strategies of genetic engineering. Definition of Genetic Engineering: The deliberate modification in genetic material of an organism by changing the nucleic acid directly is called genetic engineering. Genetic engineering holds the potential to extend the range and power of every aspect of biotechnology. The techniques will be used widely to [...]
The following points highlight the five main enzymes involved in genetic engineering. The enzymes are: 1. Restriction Endonuclease 2. DNA Ligase 3. Alkaline Phosphatase 4. DNA Polymerase and the Klenow Fragment 5. Reverse Transcriptase. Genetic engineering became possible with the discovery of mainly two types of enzymes: the cutting enzymes called restriction endonucleases and the joining enzymes called ligases. Restriction [...]