Object: To separate leaf pigments by column chromatography. or To separate the chloroplast pigments by solvent method. Requirements: Pigments extract, magnesium oxide, filter glass disc, glass wool, flask, suction pump, chromatographic tube, petroleum ether, benzene. Method: 1. Take a chromatographic tube having a filter glass disc and glass wool at its base. 2. Fill the chromatographic tube with magnesium oxide. [...]
The below mentioned article includes a list of three experiments on permeability. 1. Experiment to study the rates of penetration (permeability) of various organic solvents into living epidermal cells: Requirements: Onion bulbs, slides, cover slip, microscope, water, stop watch, glucose, sucrose, glycerol, methyl alcohol, tertiary butyl alcohol (TBA). Method: 1. Take an onion bulb and peel off a large segment [...]
The below mentioned article includes a collection of seven experiments on enzyme activity. 1. Experiment to demonstrate the activity of enzymes: Requirements: Benzidine solution, razor, thin sections of actively growing root (or germinating seeds or germinating pollen grains), phosphate buffer, hydrogen peroxide (1%), ammonium chloride (5%), starch paste. Method, observations and results: 1. Cut thin sections of fast-growing roots or [...]