Sometimes it has been found that when a series of reactions is catalysed by a number of enzymes in sequence, the accumulation of the final end-product may cause inhibition in the activity of the first enzyme of the series. This inhibition due to a compound (final end product) which is totally different in structure from the substrate of the enzyme [...]
The following points highlight the seven factors affecting the activity of enzymes in plants. The factors are: 1. Temperature 2. Hydrogen Ion Concentration 3. Water 4. Concentration of the Substrate 5. Enzyme Concentration 6. Inhibitors and 7. Accumulation of End-Products. Factor # 1. Temperature: Usually the activity of the enzymes is optimum at normal body temperature. At very low temperature [...]
In this article we will discuss about the Michaelis-Menten Constant and Significance of Michaelis-Menten Constant. Michaelis-Menten Constant: In an enzyme catalysed reaction when there is large excess of substrate and the enzyme concentration is held constant, if substrate concentration (S) is plotted against velocity (V) or reaction rate, a hyperbolic curve is obtained (fig. 10.13). This type of plot is [...]