The work of J. Cairns demonstrated DNA replication in bacteria in an elegant way. He used high resolution autoradiography for observing the distribution of radioactive label in the "chromosomes" of dividing E. coli cells. The bacterial cells were grown on a medium containing tritiated thymidine. The labelled DNA was extracted from the cells and spread on EM grids. A very [...]
The Watson-Crick model of DNA has been the basis for further work on DNA replication, mutation and recombination. Much evidence exists to establish the validity of this model. Nevertheless, the model has been scrutinized by other workers, and from time to time other models have been suggested by Wu (1970), Arnott (1968), Wang (1979) and a few others. Wang's model [...]
In this article we will discuss about:- 1. Definition of DNA Replication 2. Enzymes Involved In DNA Replication 3. Mechanism 4. Models 5. Watson and Crick's Model. Contents: Definition of DNA Replication Enzymes Involved In DNA Replication Mechanism of DNA Replication Models for DNA Replication Watson and Crick's Model for DNA Replication 1. Definition of Replication of DNA: One of [...]