After reading this article you will learn about the structure and mechanism of ATP synthase, with the help of suitable diagrams. Boyer and Walker received the Nobel Prize in 1997 for elucidating the mechanism of ATP synthase. This is all-important reactions in which the proton-motive force, produced by proton translocation, is coupled to the synthesis of ATP from ADP and [...]
The following points highlight the three methods to introduce alien DNA into host cell. The methods are:- 1. Microinjection 2. Electroporation 3. Shot-Gun/Gene Gun Method of DNA Introduction. Method # 1. Microinjection: DNA can also be stably introduced into tissue culture cells by its direct microinjection into the nuclei of the cells, using a glass micropipette that has been drawn [...]
The following points highlight the top sixteen techniques used in cell biology. Some of the techniques are: 1. Immunofluorescence Microscopy 2. Ion-Exchange Chromatography 3. Affinity Chromatography 4. Partition and Adsorption Chromatography 5. Gel Filtration Chromatography 6. Radioactive Tracer Technique 7. Radioimmunoassay (RIA) 8. Enzyme Immunoassay 9. Spectroscopy and Others. Cell Biology: Technique # 1. Immunofluorescence Microscopy: Immunological technique is the [...]