The below mentioned article provides a practical guide to microscopes, drawing appliances and measurement. Light Microscope: The different components of a light microscope are shown in Fig. 1.1. The path of light through a microscope is shown in Fig. 1.2. The most important parts of a microscope are its lenses, of which there are two — objective and eye-piece (ocular), [...]
The below mentioned article provides study notes on Microtome Sections:- 1. Section-Cutting 2. Mounting of Sections 3. Stretching of Sections 4. Drying of Sections 5. Staining the Sections 6. Staining the Sections 7. Schedule of Staining the Section. Microtome Sections # Section-Cutting: Rotary microtomes are commonly used for section-cutting. Move the block holding the socket backward as far as possible [...]
In this article we will discuss about the preparation of paraffin block. Transfer the tube or tubes containing the material to a high temperature paraffin bath (60°C), along with some paraffin of suitable melting point taken in a beaker. Suitable melting point refers to the melting point of paraffin which will give ideal ribbons of sections (see section cutting). This [...]