The below mentioned article provides a Practical Experiment on Culturing of Lambda (λ) Phage. Principle: Bacteriophage λ is cultured in E.coli cell suspension. The phage is allowed to harbour and multiply till the complete lysis of bacterial cells. Then the bacterial cells are killed with chloroform and the phage particles are separated by centrifugation. Materials: A. E. coli LE 392 [...]
In this article we will discuss about the Structure of Bacterial Cell. Bacteria (sing. bacterium) are unicellular prokaryotic microorganisms which divide by binary fission. They do not possess nuclear membrane and the nucleus consists of a single chromosome of circular double-stranded DNA helix (Fig. 1.1). Flagella: These are long filamentous, cytoplasmic appendages, 12-30 μm in length, protruding through the cell [...]
This article throws light upon the ten main types of laboratory diagnosis of bacterial diseases. The types are: 1. Diphtheria 2. Enteric Fever 3. Isolation of the Organism 4. Bacillary Dysentery 5. Diarrhoeal Diseases 6. Bacterial Food Poisoning 7. Cholera 8. Urinary Tract Infections 9. Meningitis 10. Tuberculosis. Bacterial Disease: Type # 1. Diphtheria: The genus corynebacterium comprises of: 1. [...]